Journal: eLife

Article Title: 7,8-Dihydroxyflavone modulates bone formation and resorption and ameliorates ovariectomy-induced osteoporosis

doi: 10.7554/eLife.64872

Figure Lengend Snippet:

Article Snippet: Antibody , Rabbit monoclonal anti-TAK1 , Huabio , Cat #:ET1705-14 , WB: 1:1000.

Techniques: Recombinant, Sequencing, Small Interfering RNA, Transfection, CCK-8 Assay, Quantitation Assay, Bicinchoninic Acid Protein Assay, ALP Assay, SYBR Green Assay, Enzyme-linked Immunosorbent Assay, Software

Journal: PLOS One

Article Title: TGFBR1 gene silencing attenuates cardiomyopathy in the HFpEF mouse model

doi: 10.1371/journal.pone.0328981

Figure Lengend Snippet: (A) Representative western blot images of p-TAK1 expression in the left-ventricular samples from each group of mice. (B) Quantification of the protein expression of p-TAK1 in (A) ; n = 6 per group. (C) Representative images of p-TAK1 immunohistochemistry (IHC). Bar = 200 μm; n = 6 per group. (D) Quantification of IHC of p-TAK1. (E) Representative immunoprecipitation images of TGFBR1, TAK1, and RIPK1. (F) Quantification of the binding rate of TAK1 to RIPK1; n = 4 per group. The normality of data distribution was tested using the Shapiro–Wilk method. One-way ANOVA was applied in (B) , (D) , and (F) . * p < 0.05, ** p < 0.01, and *** p < 0.001 between the indicated groups.

Article Snippet: The antibodies used for IHC analysis were as follows: collagen I (1:200, AF7001, Affbiotech), collagen III (1:200, AF0135, Affbiotech), p-TAK1 (1:200, AF4379, Affbiotech), and TRAF6 (1:200, AF5376, Affbiotech).

Techniques: Western Blot, Expressing, Immunohistochemistry, Paraffin-embedded Immunohistochemistry, Immunoprecipitation, Binding Assay

Journal: PLOS One

Article Title: TGFBR1 gene silencing attenuates cardiomyopathy in the HFpEF mouse model

doi: 10.1371/journal.pone.0328981

Figure Lengend Snippet: (A) Representative western blot images of p-TAK1, p-Smad2, and p-Smad3 in the groups of H9C2 cells. (B) Quantification of the protein expression of p-TAK1, p-Smad2, and p-Smad3 in (A) . (C) Representative western blot images of TRAF6 and p-TAK1 in the groups of H9C2 cells. (D) Quantification of the protein expression of TRAF6 and p-TAK1 in (C) . (E) Representative western blot images of TRAF6 in the groups of H9C2 cells. (F) Quantification of the protein expression of TRAF6 in (E) , and relative TRAF6 mRNA expression levels in the groups of H9C2 cells. (G) Representative immunofluorescence staining of TRAF6 (green) in the groups of H9C2 cells. The nuclei were counterstained with DAPI (blue). Bar = 50 μm. (H) Quantification of fluorescence intensity of TRAF6 in (G) ; (A) –(H) n = 6 per group. (I) Representative western blot images of TRAF6 in the left-ventricular samples from each group of mice. (J) Quantification of the protein expression of TRAF6 in (I) , and relative TRAF6 mRNA expression levels in the left-ventricular samples from each group of mice. (K) Representative images of myocardial TRAF6 immunohistochemistry (IHC). Bar = 200 μm. (L) Quantification of IHC of TRAF6; n = 6 mice per group for panels I–L. The normality of data distribution was tested using the Shapiro–Wilk method. One-way ANOVA was applied in (B) , (D) , (H) , (F) , and (L) . * p < 0.05, ** p < 0.01, and *** p < 0.001 between the indicated groups.

Article Snippet: The antibodies used for IHC analysis were as follows: collagen I (1:200, AF7001, Affbiotech), collagen III (1:200, AF0135, Affbiotech), p-TAK1 (1:200, AF4379, Affbiotech), and TRAF6 (1:200, AF5376, Affbiotech).

Techniques: Western Blot, Expressing, Immunofluorescence, Staining, Fluorescence, Immunohistochemistry

Journal: PLOS One

Article Title: TGFBR1 gene silencing attenuates cardiomyopathy in the HFpEF mouse model

doi: 10.1371/journal.pone.0328981

Figure Lengend Snippet: TGFBR1 gene silencing leads to a reduction in myocardial collagen synthesis through the Smad2/3 signaling pathway and inhibits myocardial hypertrophy in HFpEF mice through the p38/MAPK–JNK1/2 signaling pathway. Furthermore, TGFBR1 gene silencing impedes myocardial remodeling in HFpEF mice by curbing TAK1-mediated PANoptosis, predominantly because TGFBR1 gene silencing hinders the segregation of RIPK1 from TAK1 and reduces TRAF6 expression.

Article Snippet: The antibodies used for IHC analysis were as follows: collagen I (1:200, AF7001, Affbiotech), collagen III (1:200, AF0135, Affbiotech), p-TAK1 (1:200, AF4379, Affbiotech), and TRAF6 (1:200, AF5376, Affbiotech).

Techniques: Expressing